M. Phil biotechnology India

Please provide me M.Phil syllabus from Bharathiar University.

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Unit I
Introduction to research methodology:
What is research? Basic and applied research, Essential steps in research, Defining the research problem, Research/Experimental design, Literature collection, Literature citation, Research report: components, Format of thesis and dissertation,
Manuscript/research article, Review monographs, Bibliography and Reference, Significance of research.

Unit II
Biophysical methods:
Analysis of biomolecules using UV/Visible, fluorescence, circular dichroism, NMR and ESR spectroscopy, structure determination using x-ray diffraction and NMR; Analysis using light scattering, different type of mass spectrometry and urface plasma resonance methods.
Radiolabeling techniques: Properties of diff'erent types of radioisotopes normally used in biology, their detection and measurement; incorporation of radioisotopes in biological tissues and cells, molecular imaging of radioactive material safety guidelines.

UNit III
Microscopic techniques:
Visualization cells and subcellular components by light microscopy, resolving powers of different microscopes, microscopy of living cells, canning and transmission microscopes, different fixation and staining techniques for EM, freez-tech and freez- fracture methods for EM, image processing methods in microscope. Histochemical and Immunotechniques: Antibody generation, detection of molecules using ELISA, RIA, western blot, immunoprecipitaion, flowcytometry and Immune fluorescence microscopy, detection of molecules in living cells, in situ Localization such as FISH and GISH.

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