Sathyabama Institute of Science and Technology B.Sc - MicroBiology SMB4502 Mycology and Parasitology Lab Syllabus

[KunwarR]

Sathyabama Institute of Science and Technology B.Sc - MicroBiology SMB4502 Mycology and Parasitology Lab Syllabus

SATHYABAMA INSITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING

SMB4501 BACTERIOLOGY AND VIROLOGY LAB
L T P Credits Total Marks
0 0 3 2 100

SUGGESTED LIST OF EXPERIMENTS
1. Collection and processing of clinical specimen for microbiological examination.
2. Staining techniques- Simple, Negative, Gram‟s, capsule, Spores(Organism – Bacillus, Enterobacter,
Escherichia coli) Acid fast staining.
3. Study of composition and use of important differential media for identification of pathogenic bacteria:
EMB Agar, McConkey agar, Mannitol salt agar, Deoxycholate citrate agar, TCBS
4. Isolation and identification of microorganisms from urine sample(E.coli, Proteus, Pseudomonas
5. Biochemical tests for bacterial identification(IMViC, TSI, nitrate reduction, urease production,coagulase
and catalase tests.)
6. Preparation of dried filter paper discs for susceptibility assay and Antimicrobial activity by Kirby – Bauer
disc diffusion technique.
7. Determination of MIC & MBC.
8. Preservation of bacterial cultures by various techniques
9. Studying isolation and propagation of animal viruses by chick embryo technique
10. Isolation and enumeration of bacteriophages (PFU) from water/sewage sample using double agar layer
technique
11. To perform HBsAg by ELISA
12. To perform HIV Tridot method.

SMB4502 MYCOLOGY AND PARASITOLOGY LAB
L T P Credits Total Marks
0 0 3 2 100

SUGGESTED LIST OF EXPERIMENTS
1. Identification of common fungus like Mucor, Rhizopus, Penicillium, Aspergillus by Lactophenol cotton
blue mount examination.
2. Preparation of fungal media and Cultivation of fungi study of colony characters of yeast and mold.
3. Leishman staining for malarial parasites
4. Demonstration of permanent slide of Trichuris, Ascaris and Hookworm
5. Saline wet mount for observing ova and eggs of parasites.
6. Iodine wet mount for observing ova and eggs of parasites.
7. Concentration of stool samples by floatation method
8. Zinc sulphate conc. Method for stool sample
9. Demonstration of various parasites by permanent slides.
10. Concentration of stool sample by sedimentation method