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 Sathyabama Institute of Science and Technology B.Tech - BioTechnology SBTA1401 Enzyme Technology Syllabus SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF BIO AND CHEMICAL ENGINEERING SBTA1401 ENZYME TECHNOLOGY L T P Credits Total Marks 3 * 0 3 100 UNIT 1 INTRODUCTION TO ENZYMES 9 Hrs. Nature and function of enzymes, Enzyme nomenclature, classification of enzymes, mechanisms of catalysis - Acid base catalysis, electrostatic catalysis, covalent catalysis and enzyme catalysis., Role of co-enzymes and co-factors. UNIT 2 ENZYME KINETICS 9 Hrs.. Michaelis - Menton kinetics, determination of Km, Lineweaver Burke plot, Eadie - Hofstee plot, Hanes-Woolf plot. Turnover number, Enzyme inhibition -Types of enzyme inhibition, competitive inhibition, uncompetitive inhibition, non-competitive inhibition, Irreversible inhibition and inactivation kinetics. Multi substrate enzyme catalysed reaction. UNIT 3 ENZYME IMMOBILIZATION 9 Hrs. Classification of enzyme immobilization, physical and chemical techniques for enzyme immobilization -adsorption, matrix entrapment, encapsulation, cross-linking, covalent bonding, advantages and disadvantages, Application of immobilized enzymes, mass transfer effect on immobilization, properties of immobilized enzymes. UNIT 4 ENZYME SPECIFICITY AND EXTRACTION 9 Hrs. Types of specificity, Active site- Fischer hypothesis and Koshland hypothesis, Extraction of soluble enzymes, membrane bound enzymes, purification procedures, criteria of purity. UNIT 5 CLINICAL AND INDUSTRIAL APPLICATIONS OF ENZYMES 9 Hrs. Application of enzymes in medicine and industry – Assay of plasma enzyme, enzyme biosensors, design of enzyme electrodes, Synzymes, Abzymes, Enzymes in genetic engineering. Max.45 Hrs. COURSE OUTCOMES On completion of the course, student will be able to CO1 - The knowledge on enzyme and enzyme reactions will be the helpful to understand various concepts in biotechnology. CO2 - Enzyme kinetics will give inputs to use enzyme in research and industry. CO3 - Methods of enzyme immobilization help in food, pharmaceutical and chemical industries to innovate a useful product to society. CO4 - Concepts on Isolation, Purification and characterization of enzymes will be helpful to work technologically. CO5 - Application of enzymes in industry. CO6 - Identify novel applications of enzymes in bioengineering. TEXT / REFERENCE BOOKS 1. Palmer, Enzyme, Horwood Publishing Series, 2001. 2. Price and Stevens, Fundamental of Enzymology, Oxford University Press, 2002. 1. Prasad N.K., Enzyme Technology: Pacemaker of Biotechnology Paperback, 2011. 2. Khan M.Y. and Farha Khan, Principles of Enzyme Technology, 2015. 3. Helmut Uhling, Enzyme technology, John Wiley, 1998. END SEMESTER EXAMINATION QUESTION PAPER PATTERN Max. Marks: 100 Exam Duration: 3 Hrs. Part A: 10 Questions of 2 marks each - No choice 20 Marks Part B: 2 Questions from each unit of internal choice, each carrying 16 marks 80 Marks   |
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