Sathyabama Institute of Science and Technology B.Sc - BioChemistry SMB1607 Medical Laboratory techniques Syllabus

[KunwarR]

Sathyabama Institute of Science and Technology B.Sc - BioChemistry SMB1607 Medical Laboratory techniques Syllabus

SATHYABAMA INSTITUTE OF SCIENCE AND TECHNOLOGY

SMB1607 MEDICAL LABORATORY TECHNIQUES
L T P CREDITS TOTAL MARKS
4 0 0 4 100

UNIT 1 DISEASES AND ITS AGENTS 12 Hrs.
Bacterial, Viral, Fungal and Protozoan Diseases of various human body systems, Disease associated clinical samples
for diagnosis.

UNIT 2 CLINICAL TRIALS 12 Hrs.
Collection of clinical samples (oral cavity, throat, skin, Blood, CSF, urine and faeces) and precautions required. Method
of transport of clinical samples to laboratory and storage.

UNIT 3 STAINING TECHNIQUES 12 Hrs.
Examination of sample by staining - Gram stain, Ziehl-Neelson staining for tuberculosis, Giemsa stained thin blood film
for malariaPreparation and use of culture media - Blood agar, Chocolate agar, Lowenstein-Jensen medium,
MacConkey agar, Distinct colony properties of various bacterial pathogens.

UNIT 4 ASSAYS 12 Hrs.
Serological Methods - Agglutination, ELISA, immunofluorescence, Nucleic acid based methods - PCR, Nucleic acid
probes

UNIT 5 DISEASE PREDICTION 12 Hrs.
Typhoid, Dengue and HIV, Swine flu Testing for Antibiotic Sensitivity in Bacteria Importance, Determination of
resistance/sensitivity of bacteria using disc diffusion method,Determination of minimal inhibitory concentration (MIC) of
an antibiotic by serial double dilution method

TEXT BOOKS/ REFERENCE BOOKS.
1. Ananthanarayan R and Paniker CKJ (2009)Textbook of Microbiology, 8th edition, Universities Press Private Ltd.
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnick and Adelberg’s
Medical Microbiology. 26th edition. McGraw Hill Publication
3. Randhawa, VS, Mehta G and Sharma KB (2009) Practicals and Viva in Medical Microbiology 2nd edition, Elsevier
India Pvt Ltd
1. Tille P (2013) Bailey’s and Scott’s Diagnostic Microbiology, 13th edition, Mosby Collee JG, Fraser, AG, Marmion,
BP,

END SEMESTER EXAMINATION QUESTION PAPER PATTERN
Max. Marks : 100 Exam Duration : 3 Hrs.
PART A : 10 questions of 2 marks each - No choice 20 Marks
PART B : 2 questions from each unit of internal choice; each carrying 16 marks 80 Marks